Figure 2.

Autophagy is suppressed post reperfusion following mild ischemia. Rats, either Sham-operated (Sham) or subjected to 45 min of renal ischemia (I45), were sacrificed at various time points post reperfusion (R0h, R1h, R3h, R6h, R24h, R48h, R7d, and R90d). Kidney tissue was collected and analyzed by Western blotting for LC3 (A), p62 (B), S6, and phosphorylated S6 (pS6) (C), and by qPCR for the mRNA expression of LC3 (D), p62 (E), and BECN1 (F). * p <0.05, ** p < 0.01, *** p < 0.001. N = 6.