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Apoptosis increases post reperfusion following mild ischemia. Rats, either Sham-operated (Sham) or subjected to 45 min of renal ischemia (I45), were sacrificed at various time points post reperfusion (R0h, R1h, R3h, R6h, R24h, R48h, R7d, and R90d). Kidney tissue was collected and analyzed by Western blotting for Bax (A), Bcl-2 (B), and Cleaved Caspase 3 (C), and by qPCR for the mRNA expression of Bax (D), Bcl-2 (E), and Bim (F). * p <0.05, ** p < 0.01, *** p < 0.001. N = 6.
