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Isolation of breast CSC inhibitors from carrot extracts based on mammosphere formation assays. (A) The purification procedure for the mammosphere inhibitor. (B) Inhibitory effect of carrot extracts on mammosphere growth. The mammospheres were incubated with carrot extracts or DMSO. The mammospheres were photographed with a microscope at 10× magnification (scale bar = 100 µm). (C) Purified samples of carrot extracts were analyzed by HPLC chromatogram.
