Figure 2.

Effect of sauchinone on AngII-induced mesangial cell invasion and cell cycle-related proteins. (A) Microscopy images of the wound-healing assay performed on mesangial cells at 0 h and 48 h. Each photograph is representative of at least three independent experiments (magnification: X200). (B,C) Effects of sauchinone on expression of G0/G1 phase cell cycle-related proteins in mesangial cells. Expression of cell cycle-related proteins in mesangial cells was determined by Western blot analysis. (D,E) Quantitative results demonstrating that sauchinone regulated the cell cycle-related proteins in response to AngII. Each value represents the means ± S.E. of five independent experiments. ** p < 0.01, * p < 0.05 vs. control; ## p < 0.01, and # p < 0.05 vs. AngII alone.