Figure 3.

Effect of sauchinone on AngII-induced mesangial cell fibrosis. (A) Western blotting and (B) real-time PCR showed protein and mRNA levels of fibronectin, Collagen IV, and CTGF in sauchinone-treated and AngII-stimulated cells at 48 h. (C,D) Effect of sauchinone on the relative levels of TGF-β1/Smads. The results were detected by Western blotting and real-time PCR. (E) Immunofluorescent images of p-Smad-2 nuclear translocation under the laser scanning confocal microscopy are show (magnification. 400×). Nuclei were stained with DAPI (blue) and p-smad-2 was stained with Alexa Fluor 488 (green). Green fluorescence indicates localization of p-Smad-2. Respective blot data were obtained from five independent experiments. Each value represents the means ± S.E. of five independent experiments. ** p < 0.01 vs. control and ## p < 0.01, # p < 0.05 vs. AngII alone.