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Confirmation of SP-D KO in mice. DNA was extracted from tail biopsies of mice and SP-D genotypes were identified by multiplex PCR. Lane 1, GAPDH in wild-type mice; lane 2, GAPDH in SP-D KO mice; lane 3, wild-type mice (0.4 kb PCR product indicated SP-D); lane 4, SP-D KO mice; lane 5, DNA marker. SP-D, surfactant protein D; KO, knockout.
