Figure 4.

Wound healing assay of CMT-93 cells using SP-D. CMT-93 cells were plated in six-well culture cluster plates. Wounds were introduced using the 200 µl pipette tips, and SP-D was added at concentrations of 0, 5, 10, 15 and 20 µg/ml. After 24 h, cells that had migrated into the wounded areas were counted under a microscope. Five images (2.8×2.0 mm) per well were randomly captured, and the wound area was measured using ImageJ. (A) SP-D significantly suppressed the increase in CMT-93 cells compared to the control, but the effect was not dose-dependent. The data shown are mean ± SD. *P<0.05, **P<0.01 compared with the control. (B) Representative image of cells at 0 h, the scraped areas without SP-D and the scraped area with 10 µg/ml SP-D. SP-D, surfactant protein D.