Figure 5.

Cell invasion assay of CMT-93 cells using SP-D. CMT-93 cells were plated in 24-well culture cluster plates. The upper insert was coated with Matrigel and incubated for 12 h. CMT-93 cells were seeded in the upper insert. SP-D was also added to the upper insert at a concentration of 0, 5, 10, 15 and 20 µg/ml. The upper insert was placed in the lower outer well with DMEM. The set was incubated at 37°C with 5% CO₂ for 36 h. Cells that passed through the Matrigel were stained using the Diff-Quick method and counted with a microscope. Invasion ability was defined as the ratio of the number of cells that passed through the Matrigel to the number of cells that could not pass through the Matrigel. (A) SP-D significantly decreased the number of CMT-93 cells that passed through the membrane compared to untreated cells (SP-D 5, 10 µg/ml). The data shown are the mean ± SD. *P<0.05 compared with the control. (B) Representative images of the stained cells (purple) that passed through the membrane without SP-D and images of the stained cells (purple) treated with 10 µg/ml SP-D that passed through the membrane. SP-D, surfactant protein D.