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. 2020 Oct 5;20(6):322. doi: 10.3892/ol.2020.12185

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Copyright: © Tajima et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 9. — Cell invasion assay of CMT-93 cells and CMT-93 PM using SP-D. (A) Both CMT-93 PM cell lines showed resistance to treatment with 10 µg/ml of the SP-D protein, whereas SP-D suppressed the invasion ability of CMT-93 cells. CMT-93 PM (2) enhanced the invasion capacity due to the SP-D treatment (P=0.016). The data shown are mean ± SD. *P<0.05, **P<0.01 compared with the control. (B) Representative images of the stained CMT-93 cells (purple) that passed through the membrane treated without SP-D and with SP-D, and images of the stained CMT-93 PM (2) cells (purple) that passed through the membrane treated without SP-D and with SP-D. SP-D, surfactant protein D; CMT-93 PM, CMT-93 pulmonary metastases.