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. 2020 Sep 30;25(19):4499. doi: 10.3390/molecules25194499

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of ST09 on cell migration: (A) ST09 treated MDA-MB-231 cells in vitro subjected to migration on Matrigel. (B) Quantification of migrated cells is represented as a scatter plot. (C) An immunoblot of MMP1, MMP2, and Vimentin proteins was performed from MDA-MB-231 cell lysate treated with the ST09 compound for 48 h. Quantification was done for each protein and is represented as a bar graph of mean +/− SEM. Every experiment was performed twice, and a two-way ANOVA test was used to calculate the significance between control and ST09 treated groups. Significance for immunoblots was calculated using a t-test and one-way ANOVA to calculate p-value between control and ST09 treated samples. (* p < 0.05, *** p < 0.0001).