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. 2020 Sep 25;21(19):7062. doi: 10.3390/ijms21197062

Figure 3.

Figure 3

Effect of joint knock down of Pkm1 and of Pkm2 on C2C12 myotube size. (A) Pkm1 and Pkm2 mRNA in C2C12 myotubes following 24 h IGF-1 treatment. (B) PKM2 protein abundance in IGF-1 treated C2C12 myotubes. (C,D) Pkm1 and Pkm2 mRNA expression following siRNA interference of Pkm (n = 5). (E,F) C2C12 myotube diameter (n = 6). Black circles indiciate individual data points. Scale bar is 100 μm. * Significantly different between indicated conditions, unpaired t-test, Mann–Whitney U test or two-way ANOVA with Bonferroni post hoc test (p < 0.05); ** (p < 0.01); *** (p < 0.001).