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. 2020 Sep 23;21(19):6991. doi: 10.3390/ijms21196991

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of 7 rh on chondrocyte apoptosis in articular cartilage. (A) TUNEL staining in the articular cartilages of the sham control groups (sham+ saline and sham+ 7 rh 13.8 nM), ACLT, and two 7 rh treating groups. The arrows mean positive stain of TUNEL. (B) The representative micrographs of immunolocalized activated caspase 3. (C) Quantitative analysis of the apoptotic rate. (D) Quantitative analysis of the immunohistochemical staining of activated caspase 3. Each bar represents the mean±SEM of 12 samples in each group. (** p < 0.01 versus the sham control groups; *** p < 0.001 versus the sham control groups; ^# p < 0.05 versus the ACLT group; ^### p < 0.001 versus the ACLT group).