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. 2020 Oct 5;21(19):7352. doi: 10.3390/ijms21197352

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PSTVd infection differentially affects the expression of six expansin genes of tomato. Total RNA extracted from tomato plants inoculated with LMW-RNA of PSTVd-D_wt, PSTVd-I_wt and mutants of PSTVd-I (PSTVd-I:C42U/64U, PSTVd-I:C42U, and PSTVd-I:64U) at 3-wpi were assayed by RT-qPCR to analyze the expression level of (A) EXPA2, (B) EXPA5, (C) EXPA9, (D) EXPA11, (E) EXPA14, and (F) EXPA18 mRNAs. The vertical axis indicates the relative expression level (Log2) of mRNAs. The data were normalized by the mean value of the mock-inoculated control at 1-wpi as 1.0. A t-test was performed with a p-value of 0.05. The plants that showed a difference < 0.05 and <0.01 are indicated by lower and capital letters on the top of the bars, respectively. In the figure, significant difference against the mock-inoculated control plant, PSTVd-D_wt, PSTVd-I:C42U/64U, PSTVd-I:C42U, and PSTVd-I:64U is represented as a or A, b or B, c or C, d or D, and e or E, respectively. Mean values are based on three biological and three technical replicates.