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. 2020 Sep 30;25(19):4493. doi: 10.3390/molecules25194493

Figure 2.

Figure 2

Mu and delta opioid receptors co-localize in the lysosomal compartment upon CYM51010 activation in primary hippocampal cultures. (A) Representative confocal images showing mu-mCherry–delta-eGFP colocalization with LAMP1 immunoreactive compartment under basal conditions or 60 min after CYM51010 application (400 nM). Scale bar = 10 μm (inset scale bar = 2.5 μm). (B) Drug treatment induces statistically significant increase in the amount of colocalization of mu-mCherry/delta-eGFP colocalization with LAMP1 labeling. Two-way ANOVA Fdrug treatment (1, 49) = 62.70; p < 0.0001. Freceptor (1, 49) = 2.12, p = 0.15; Finteraction (1, 49) = 3.65, p = 0.2. Tukey’s post hoc test: *** p < 0.001 for both mu-mCherry and delta-eGFP; n = 10–20 neurons per group from at least three independent cultures.