Table 2.

Analytical parameters of the calibration curves obtained from individual and multianalyte immunoassays on gold SPE 8x compared with the parameters previously evaluated by ELISA [11], fluorescent microarray [31] and individual SPE in 1× format [32].

	ELISA [11] *1	Microarray [31] *1	SPE 1x Format [32] *2	SPE 8× Format *1	Multianalyte SPE 8× Format *2	ELISA [11]	Microarray [31]	Individual SPE 8× Format	Multianalyte SPE 8× Format
	Irgarol 1051					Sulfapyridine
Signalmax	100.4 ± 0.4	100.6 ± 2.1	95.42 ± 4.40	92.2 ± 6.0	97.3 ± 6.5	100.4 ± 0.6	97.1 ± 5.1	90.9 ± 10.0	91.3 ± 4.3
Signalmin	2.9 ± 0.4	3.1 ± 1.9	−2.45 ± 4.78	−0.6 ± 5.2	23.3 ± 8.3	2.7 ± 1.2	−2.5 ± 6.4	−0.22 ± 8.4	9.9 ± 7.0
Slope	−0.98 ± 0.22	−1.45 ± 0.19	−0.81 ± 0.16	−1.07 ± 0.26	−5.01 ± 4.9	−0.784 ± 0.09	−1.14 ± 0.36	−0.93 ± 0.37	−3.50 ± 1.51
IC50, nM	0.58 ± 0.19	2.29 ± 1.11	4.12 ± 1.31	4.77 ± 1.27	19.89 ± 1.16	6.58 ± 1.73	15.23 ± 1.34	5.65 ± 1.57	13.85 ± 1.20
IC50 µg/L	0.145 ± 0.05	0.579 ± 0.28	1.04 ± 0.33	1.20 ± 0.23	5.04 ± 0.29	1.43 ± 0.23	3.79 ± 0.33	1.40 ± 0.39	3.45 ± 0.29
LOD µg/L	0.012 ± 0.007	0.135	0.038 ± 0.022	0.037	3.24	0.08 ± 0.02	0.397	0.009	1.08
LOQ µg/L	-	-	0.24 ± 0.23	0.207	3.97	-	-	0.16	2.05
R2	0.999	0.997	0.98	0.85	0.77	0.999	0.983	0.77	0.89
	Chloramphenicol					Estradiol
Signalmax	102.6 ± 8.8	98.8 ± 2.8	-	98.2 ± 3.8	97.2 ± 6.5	99.8 ± 0.5	97.7 ± 1.7	89.9 ± 6.7	96.1 ± 4.3
Signalmin	8.8 ± 1.2	28.7 ± 3.9	-	1.5 ± 3.4	20.0 ± 10.5	0.6 ± 0.7	−0.05 ± 2.39	0.45 ± 8.2	20.4 ± 6.5
Slope	−0.60 ± 0.05	−1.18 ± 0.32	-	−1.30 ± 0.19	−2.86 ± 1.99	−1.01 ± 0.07	−1.23 ± 0.15	−1.32 ± 0.62	−9.56 ± 4.3
IC50, nM	0.59 ± 0.05	10.25 ± 1.28	-	3.47 ± 1.15	12.91 ± 1.36	4.01 ± 0.74	9.67 ± 1.11	35.4 ± 1.36	21.83 ± 1.05
IC50 µg/L	0.192 ± 0.09	3.00 ± 0.41	-	1.12 ± 0.37	4.17 ± 0.44	1.09 ± 0.20	2.63 ± 0.30	9.72 ± 0.37	5.94 ± 0.28
LOD µg/L	0.004 ± 0.01	0.453	-	0.18	1.89	0.11 ± 0.03	0.362	0.41	4.60
LOQ µg/L	-	-	-	0.36	2.70	-	-	1.93	5.18
R2	0.997	0.988	-	0.94	0.72	0.999	0.998	0.90	0.82

*¹ PBST; *² sea water.