Figure 3.

Impairment of mitophagy in the mouse auditory cortex with aging. (A) Colocalization analysis of autophagosomes and mitochondria. Immunofluorescence analysis revealed that the colocalization (yellow puncta, the overlap color) of LC3B (green) and TOM20 (red) in the mouse auditory cortex significantly decreased with aging. (B) Colocalization analysis of lysosomes and mitophagosomes. Immunofluorescence analysis revealed that the colocalization (yellow puncta, the overlap color) of LAMP1 (green) and TOM20 (red) in the mouse auditory cortex significantly decreased with aging. The data are shown as mean ± standard error of mean (five mice per group; 1 m, 1 month; 6 m, 6 months; 12 m, 12 months; 18 m, 18 months; DAPI, 4′,6-diamidino-2-phenylindole). * p < 0.05, ** p < 0.01, ns: not significant.