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. 2020 Oct 7;20(6):334. doi: 10.3892/ol.2020.12197

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Copyright: © Song et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Differential analysis of GNG4 expression in Chinese patients with COAD. (A) Transcriptional expression levels of GNG4 in normal controls and patients with COAD. The raw data were obtained from the Oncomine database. Data were analyzed with GraphPad Prism using two-tailed t-tests (unpaired). (B) Differential GNG4 mRNA expression between LCC and RCC was determined using reverse transcription-quantitative PCR. (C) Immunohistochemical staining of GNG4 in normal, LCC and RCC tissues in the tissue microarray. (D) Protein expression levels of GNG4 in normal, LCC and RCC tissues, as measured by western blotting. The graph presents the relative protein expression levels of GNG4 in the aforementioned three tissues (one-way ANOVA, followed by Tukeys post hoc test). *P<0.05; **P<0.01. COAD, colon adenocarcinoma; GNG4, guanine nucleotide binding-protein γ subunit 4; LCC, left-sided colon cancer; RCC, right-sided colon cancer.