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. 2020 Sep 29;21(19):7196. doi: 10.3390/ijms21197196

Figure 3.

Figure 3

All ADs improve colony-forming capacity of DKC-derived BM-MNCs and danazol and oxymetholone increase the expression of TERT in PBMC of DKC patients. (A) BM-MNCs were cultured in a semisolid medium in presence of the highest doses of ADs depicted in the previous figure (DN 150 nM, OX 1 µM and ND 5 µM) versus DMSO, in order to perform a CFU assay. The number of colonies was assessed after 7 days and normalized towards the control value. One sample t-test was used to determine statistical significance. This experiment was performed with three different patient samples in independent experiments and results are showing their mean values ± SD. Patient #1 is represented with a circle, Patient #2 with a triangle and patient #3 with a square. (B) TERT mRNA levels in colonies obtained from the CFU assay of patient #1 were determined. Values were normalized towards DMSO values and one sample t-test was used to determine statistical significance. (C) TERT mRNA levels were determined in PBMC treated with ADs at different concentrations for 7 days (DN 37.5, 75 and 150 nM; OX 0.25, 0.5 and 1 µM; ND 1.25, 2.5 and 5 µM ). TERT levels were normalized towards DMSO values and one sample t-test was used to determine statistical significance. This experiment was performed with two different patient samples (patient #1, circle and #2, triangle) in independent experiments and results are showing their mean values ± SD. * p < 0.05, ** p < 0.01.