Fig. 5.

Canonical thermogenesis does not underlie the metabolic protection observed in the adult Phospho1-deficient mice. Brown adipose tissue (BAT) mass in a juvenile (35-day-old) and b adult (120-day-old) WT and Phospho1^−/− mice. c Representative micrographs of BAT from WT and Phospho1^−/− mice. d Brown fat gene expression and e UCP1 protein analysis. f–i Insulin sensitivity and protection from diet induced obesity in Phospho1^−/− mice was not a consequence of altered energy expenditure (RER—respiratory exchange ratio; H4 = H3 (W)/lean mass (kg)). Data are represented as mean ± S.E.M. *p < 0.05, **p < 0.01, ***p < 0.001. Different letters above the error bar show significant difference at p < 0.05 and the data were assessed as follows: a, d two-tailed Mann–Whitney test. b, d, f–i Two-way ANOVA with multiple comparisons