Fig. 6.

Osteocalcin-independent mechanism of PHOSPHO1-regulated energy metabolism. To assess the relative change in Esp mRNA expression in primary calvarial osteoblasts, RT-qPCR was conducted to compare a primary WT and Phospho1^−/− osteoblasts and b Phospho1^−/− osteoblast transfected with empty (EV) or overexpressing (OE) vectors. c, d Osteocalcin content of serum from WT and Phospho1^−/− mice at 60 and 120 days of age. Data are represented as mean ± S.E.M n = 5–6 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001 and the data were assessed as follows: a–c Two-tailed Mann–Whitney test or two-tailed unpaired t test