Table I.

List of diplostomid species used in our phylogenetic analyses of 28S rDNA and COI mtDNA including their host species, geographical origin of material, morphological voucher numbers, and GenBank accession numbers. CNHE: Colección Nacional de Helmintos, Instituto de Biología, Universidad Nacional Autónoma de México, Mexico City. Specimen 98.01_BLV is deposited in the collection of the Biodiversity Institute of Ontario.

Digenean taxa	Host species	Geographic origin	Museum no.	Accession no.		Reference
				28S	COI
Ornithodiplostomum scardinii	Scardinius erythrophthalmus	Czech Republic	−	KX931427	KX931425	Stoyanov et al., 2017
Posthodiplostomum centrarchi	Ardea herodias	Canada	98.01_BLV	−	MH581291	Locke et al., 2018
Uvulifer ambloplitis	Megaceryle alcyon	U.S.A.	HWML-139982	MK874320	MK871329	Present study
Uvulifer batesi n. sp.	Chloroceryle inda	Peru	HWML-139983, HWML-139984	MK874321	MK871330	Present study
Uvulifer elongatus	Megaceryle torquata	Lábrea, Brazil	−	MK874322	MK871331	Present study
U. elongatus	M. torquata	Pantanal, Brazil	HWML-139985	MK874323	MK871332	Present study
Uvulifer pequenae n. sp.	C. inda	Peru	HWML-139986, HWML-139987	MK874324	MK871333	Present study
Uvulifer prosocotyle	M. torquata	Pantanal, Brazil	HWML-139988	MK874325	MK871334	Present study
Uvulifer spinatus	Poecilia mexicana	Mexico	CNHE: 10322−10324	MF568582	−	López-Jiménez et al., 2018
Uvulifer weberi	Chloroceryle americana	Pantanal, Brazil	HWML-139989	MK874326	MK871335	Present study
Uvulifer sp.	Lepomis gibbosus	Canada	−	−	MF124281	Blasco-Costa and Locke, 2017
Uvulifer sp.	M. alcyon	Mexico	−	MF398332	−	Hernández-Mena et al., 2017
Uvulifer sp.	M. alcyon	Mexico	−	MF568569	−	López-Jiménez et al., 2018
Uvulifer sp.	Poecilia sp.	Mexico	−	MF568674	−	López-Jiménez et al., 2018
Uvulifer sp.	Amatitlania nigrofasciata	Mexico	−	MF568575	−	López-Jiménez et al., 2018
Uvulifer sp.	Tilapia sparrmanii	South Africa	−	MK604825	−	Hoogendoorn et al., 2019
Uvulifer sp.	Perca flavescens	U.S.A.	−	MK874327	MK871336	Present study