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. 2020 Oct 23;15(10):e0241192. doi: 10.1371/journal.pone.0241192

Fig 1. Transglutaminase protein and activity are observed in mouse mammary epithelial cells.

Fig 1

A. Brightfield image of non-lactogenic HC11 cells, not treated with prolactin or hydrocortisone B., and lactogenically differentiated HC11 cells show morphological changes in the form of mammosphere production seen by the arrows. C. Whey acidic protein (Wap) gene expression is upregulated when treated with lactogenic hormones and further increased with lactogenic hormones + 5HTP. D. Western blot analysis of transglutaminase 2 (TG2) in lysate of HC11 cells before differentiation (proliferation media) and after differentiation (lactogenic media), quantified in E., show no change in protein expression. TG2 quantification relative to the actin control. F. TG2 gene expression is unchanged before and after differentiation. G. TG activity significantly increases overtime in the lactogenic differentiated cells. Values are means and bars indicate SEM (n = 3 independent cell cultures). *p<0.05 **p<0.01 ***p<0.001 ****p<0.0001.