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. 2020 Oct 23;15(10):e0233938. doi: 10.1371/journal.pone.0233938

Fig 9. Effect of OBE on the expression/activity of pro-inflammatory molecules and immune cell markers, and neutrophil superoxide release and spontaneous apoptosis in vitro.

Fig 9

Colon sections taken from mice treated for with DSS/vehicle, DSS/OBE (100–200 mg/kg) or untreated (UT) mice were immunostained with antisera against phosphorylated/total ERK1/2, AKT, p38, LY6G, F4/80, or actin (Panel A). The blots represent one of 3 similar experiments. Neutrophils were isolated from naïve mice and the level of superoxide release was determined by recording chemiluminescence emanating from oxidation of luminol substrate. Neutrophils were treated with vehicle only (solid circles), or 10 μM WKYMVm plus vehicle (red squares) or various concentrations of OBE (panel B). The % of viable (panel C) and % apoptotic (panel D) neutrophils in response to vehicle (open bar) or various concentrations of OBE (hatched bars) was determined using Annexin-V/7AAD assay. Histobars represent means ± SEMs for 3 independent experiments conducted with cells isolated from 3 mice in each group. Asterisks denote significant difference from vehicle group, with p<0.05.