Table 2. Primers and probes for protocols in qPCR probes for TCD organisms.
| Primer pair and probe | Length (bp) | Sequence | Position | Product size (bp) | Sequence |
|---|---|---|---|---|---|
| Geosmithia morbida | |||||
| Gmorb_228_F | 18 | GGAGATGGCGTCTCTTTG | 228 to 246 | ||
| Gmorb_319_R | 18 | ACGAGAGTCAGTGTTCTG | 319 to 301 | 92 bp | KJ148218.1 |
| Gmorb_255_P | 23 | FAM—TCTACCTCTTCCTGTCCAGCCTA—BHQ1 | 255 to 278 | ||
| Pityophthorusjuglandis | |||||
| Pjug_253_F | 22 | TCCCACGTCTTAATAATATAAG | 253 to 275 | KX809936.1 | |
| Pjug_435_R | 20 | CTCCTGCTATATGAAGACTA | 435 to 415 | 183 bp | |
| Pjug_281_P | 27 | Hex_ACTCTTACCACCATCATTAACATTCCT_BHQ1 | 281 to 308 | ||