Table 4. Analitycal sensitivity (LoD) assays.
| Dilutions 1:5 | qPCR probe G. morbida | qPCR probe P. juglandis |
|---|---|---|
| Cq means ± SD | Cq means ± SD | |
| 10 ng/μL | 25.54 ± 0.48 | 18.31 ± 1.15 |
| 2.0 ng/μL | 27.52 ± 0.43 | 20.69 ± 0.67 |
| 0.4 ng/μL | 29.57 ± 0.47 | 23.05 ± 0.34 |
| 0.08 ng/μL | 31.12 ± 0.14 | 24.57 ± 0.21 |
| 0.016 ng/μL | 33.05 ± 0.53 | 26.85 ± 0.47 |
| 3.2 pg/μL | 34.75 ± 0.19 | 28.35 ± 0.43 |
| 0.64 pg/μL | - | 30.16 ± 0.17 |
| 0.128 pg/μL | - | 32.30 ± 0.05 |
| 25.6 fg/μL | - | 33.53 ± 0.64 |
Analitycal sensitivity (LoD) assays using 1:5 serial dilutions (from 10 ng/μL to 25.6 fg/μL). Mean Cq±SD = mean of the three threshold cycles of each dilution (Cq) ± standard deviation (SD). Cq values above 35 were considered as negative results.