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. 2020 Oct 13;16(10):e1008996. doi: 10.1371/journal.ppat.1008996

Fig 5. sirt-4 overexpression is associated with reduced Wolbachia density in the ovaries.

Fig 5

One-day old virgin wMelCS-infected female flies had their ovaries dissected, DNA extracted and levels of Wolbachia quantified for the wsp gene relative to host RpL32 using SYBR Green in both (A) sirt-4 overexpression—OE (control–green: “Act5cGAL4 >“ vs. sirt-4 OE–orange: “Act5cGAL4 > UAS sirt-4 OE”) and (B) sirt-4 knockout—KO (control–green: FM6/ sirt-4 KO vs. sirt-4 KO–orange: sirt-4 KO/sirt-4 KO) scenarios. sirt-4 overexpression, but not the knockout, significantly reduced Wolbachia levels. Data represent two biological replicate experiments of randomly sampled flies. Raincloud plots depict median relative wsp levels with P-values determined for all pairwise comparisons via Kruskal-Wallis on entire dataset followed by Mann-Whitney Dunn’s-corrected test for pairwise comparisons. Each dot represents a pool of 5 pairs of ovaries. Sample size is depicted in parenthesis for each group.