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. 2020 Sep 8;98(10):skaa302. doi: 10.1093/jas/skaa302

Table 4.

Effects of steam conditioning time for flaked corn on starch availability, ISDMD, and production of fermentative gasses by in vitro cultures of mixed ruminal microbes (IVGP)

Conditioning time, min P-value
Item 15 30 45 SEM Linear Quadratic
Starch availability, %1 48.5 51.5 47.9 0.60 0.52 <0.01
ISDMD, %2 36.6 44.4 42.0 2.66 0.15 0.12
IVGP, mL3 102.3 121.3 104.9 7.33 0.57 <0.01

1Measured using the refractive index method (Sindt et al., 2006b) once per sample (90 observations, 30 per treatment) shortly after flaking.

2Measured over 3 d using six ruminally fistulated steers by incubating 2.5 g (DM) corn flakes in Dacron bags ruminally for 14 h (270 observations, 90 per treatment).

3Mean gas production during 24-h incubation period. Measured by incubating 3 g (DM) flaked corn as substrate, with 10 mL ruminal fluid inoculum and 140 mL McDougall’s Buffer at 39 °C. Repeated with two replicates per sample (90 samples, 180 observations, 60 per treatment).