Table 2.
Ingredient and calculated composition of experimental diets (as-fed basis)1
| Item | Dietary treatment | |
|---|---|---|
| Control | Test | |
| Ingredient, % | ||
| Corn | 48.5 | 48.5 |
| SBM | 20.0 | 20.0 |
| Dried whey | 17.2 | 17.2 |
| Fish meal | 10.0 | 10.0 |
| Choice white grease | 1.50 | 1.50 |
| Ground limestone | 0.50 | 0.50 |
| Sodium chloride | 0.50 | 0.50 |
| Vitamin and mineral premix2 | 0.30 | 0.30 |
| Choline chloride | 0.07 | 0.07 |
| l-Lys HCl | 0.25 | 0.25 |
| dl-Met | 0.05 | 0.05 |
| l-Thr | 0.05 | 0.05 |
| l-Trp | 0.04 | 0.04 |
| Wild-type algal protein | 1.00 | — |
| Osteopontin-enriched algal protein | — | 1.00 |
| Calculated composition 3 | ||
| ME, kcal/kg | 3,389 | 3,389 |
| CP, % | 22.3 | 22.3 |
| SID amino acids, % | ||
| Lys | 1.51 | 1.51 |
| Met + Cys | 0.78 | 0.78 |
| Trp | 0.27 | 0.27 |
| Thr | 0.85 | 0.85 |
| Val | 0.96 | 0.96 |
1All pigs received allotted treatment diet upon starting –10 DPI. Abbreviations: DPI, day postinoculation; WA, wild-type algal protein (i.e., control diet); OA, OPN-type algal protein (i.e., test diet); SID, standardized ileal digestibility.
2Vitamin–mineral premix (JBS United, Sheridan, IN) included the following per kilogram complete diet: Vitamin A (retinyl acetate), 11,128 IU; Vitamin D3 (cholecalciferol), 2,204 IU; vitamin E (dl-α tocopheryl acetate), 66 IU; vitamin K (menadione nicotinamide bisulfite), 1.42 mg; thiamine (thiamine mononitrate), 0.24 mg; riboflavin, 6.58 mg; pyridoxine (pyridoxine hydrochloride), 0.24 mg; vitamin B12, 0.03 mg; d-pantothenic acid (d-calcium pantothenate), 23.5 mg; niacin (nicotinamide and nicotinic acid), 44 mg; folic acid, 1.58 mg; biotin, 0.44 mg; Cu (copper sulfate), 10 mg; Fe (iron sulfate), 125 mg; I (potassium iodate), 1.26 mg; Mn (manganese sulfate), 60 mg; Se (sodium selenite), 0.3 mg; and Zn (zinc oxide), 100 mg.
3Metabolizable energy and standardized ileal digestible (SID) amino acid values were calculated using NRC (2012). Analyzed crude protein determined as total nitrogen × 6.25.