Table 6.
Effects of dietary algal protein supplementation and ETEC infection on prevalence of fecal bacterial gene expression (raw Ct values) in weanling pigs1
| Uninfected | ETEC-infected | Model P-value3 | |||
|---|---|---|---|---|---|
| Item2 | NC | PC | OA | SEM | DPI × TRT |
| DPI 0 | |||||
| Total bacteria (rrs) | 13.75a | 12.21b | 13.64a | 0.37 | 0.001 |
| Total E. coli (gadAB) | 35.64 | 36.68 | 32.96 | 1.34 | 0.151 |
| F18 ETEC (fedA) | ND | ND | ND | — | — |
| DPI 7 | |||||
| Total bacteria (rrs) | 13.64 | 13.53 | 12.98 | 0.43 | 0.468 |
| Total E. coli (gadAB) | 37.23a | 24.49b | 26.19b | 1.50 | <0.001 |
| F18 ETEC (fedA) | ND | 33.35 | 35.20 | 0.90 | 0.673 |
1Values represent least square means of 6 to 8 pigs per treatment. All pigs were started on their assigned dietary treatment starting at –10 DPI. Experimental treatments: NC, sham-inoculated pigs receiving 1.0% wild-type algal protein; PC, ETEC-inoculated pigs receiving 1.0% wild-type algal protein; OA, ETEC-inoculated pigs receiving 1.0% OPN-enriched algal protein. Abbreviations: ETEC, enterotoxigenic E. coli; DPI, days postinoculation; TRT, treatment; Ct, cycle threshold; ND, nondetectable.
2Bacterial gene name shown in parentheses. Data shown as raw Ct values, with higher values denoting lower expression of a particular gene.
3P-values represented were calculated on log2 transformed data.
abMeans without a common superscript letter differ (P < 0.05).