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. 2020 Sep 25;59(40):3844–3855. doi: 10.1021/acs.biochem.0c00705

Figure 5.

Figure 5

(a) Schematic enthalpy diagram for the AHA-catalyzed reaction using the values obtained from fitting to the equilibrium model of eq 3. (b) Corresponding free energy diagram in the temperature range between 283 K (blue) and 323 K (red), where it can be seen that the lowest-energy reactant state shifts from ES to ES′ around room temperature.