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(A) Effect of auransterol on cell cycle distribution in HT-29 colon cancer cells. Cells were treated for 24 h with auransterol (0–50 μM). Data represent percentage of cell distribution in each cell cycle phase. (B) Protein expression of p53 and (C) p21. Paclitaxel (0.01 μM) was used as positive control. Data are expressed as the means ± SEM (n = 3) and analyzed by one-way ANOVA using Dunnett’s multiple-comparison test (*P < 0.05, **P < 0.01) of three independent assays.
