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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Exp Eye Res. 2020 Feb 8;193:107961. doi: 10.1016/j.exer.2020.107961

Fig. 2.

Fig. 2.

Flat-mounted hemiretina sections were labeled for the RGC marker Brn3a (A). Six 40x images from each hemiretina were analyzed (bottom; white squares). RGCs were counted by a masked observer. Representative images are shown of the DFP-treated and control eyes (top). RGC density was preserved for the microbead-injected eyes of the DFP-treated mice despite elevated IOP (C, top). Error bars = Mean ± SEM; ANOVA with Tukey’s HSD **p < 0.01. The number of RGC axons was counted in optic nerve cross sections 1.5mm posterior to the globe (B). One central and 4 peripheral 75 × 75 μm images were sampled for each cross section (bottom; white squares). Higher magnification representative images (50 × 50 μm) are shown of the DFP-treated and control ONs (top). Axon swelling (arrow), gliosis (asterisk), and astrocyte activation (cross) were prominent in the microbead-injected eyes of the no DFP ONs. The AxonJ Image Analysis Algorithm plugin for ImageJ was used to count the number of axons. Axon density was preserved in the microbead-injected eyes of the DFP-treated mice despite elevated IOP (C, bottom). *p < 0.05.