Table 4.

Effect of Bacillus subtilis PB6 and/or chromium propionate supplementation on complete blood count variables^a of beef cattle during the feedlot receiving period

	Treatmentb					P-value
Itemc	CON	CST	CHR	CST + CHR	SEMd	CST	CHR	CST × CHR
Red blood cells, M/μL	9.55	9.75	9.44	9.82	0.23	0.14	0.93	0.66
Hemoglobin, g/dL	12.90a	12.52ab	12.06b	12.50ab	0.23	0.88	0.05	0.06
Hematocrit, %	39.93a	37.94b	37.31b	38.07b	—	0.37	0.07	0.05
Mean corpuscular volume, fL	44.96a	40.05b	38.35b	39.50b	1.31	0.03	<0.01	<0.01
Mean corpuscular hemoglobin, pg	14.57a	13.37b	12.88b	12.92b	0.38	0.02	<0.01	0.01
Mean corpuscular hemoglobin concentration, g/dL	32.37	33.03	32.38	32.91	0.12	<0.01	0.64	0.57
Platelets, K/μL	431.37	464.89	442.15	475.75	22.40	0.15	0.63	1.00
White blood cells, K/μL	10.93	10.38	11.60	11.23	0.37	0.20	0.04	0.81
Neutrophils, K/μL	3.23	2.90	3.72	3.56	0.21	0.27	0.02	0.69
Lymphocytes, K/μL	5.82	5.60	5.74	5.80	0.18	0.67	0.74	0.44
Monocytes, K/μL	1.76	1.76	1.90	1.76	0.09	0.39	0.40	0.41
Eosinophils, K/μL	0.12	0.18	0.13	0.16	0.03	0.17	0.90	0.71
Basophils, K/μL	0.0027	0.0024	0.0023	0.0014	0.0006	0.33	0.26	0.54
Neutrophils, %	28.11	26.91	30.01	29.71	—	0.53	0.05	0.71
Lymphocytes, %	53.93	54.09	52.17	52.74	—	0.76	0.19	0.86
Monocytes, %	16.86	17.28	16.76	16.11	—	0.84	0.27	0.35
Eosinophils, %	1.12	1.64	1.13	1.41	—	0.16	0.68	0.67
Basophils, %	0.0240	0.0250	0.0219	0.0129	—	0.40	0.17	0.50
Neutrophil:lymphocyte	0.58	0.55	0.67	0.63	0.04	0.39	0.03	0.82

^aComplete blood count analysis was conducted using ProCyte Dx Hematology Analyzer, IDEXX Laboratories, Westbrook, ME.

^bCON = control; CST = 13 g per animal daily DM inclusion of prepared Bacillus subtilis PB6 product (CLOSTAT, Kemin Industries, Des Moines, IA); CHR = 450 ppb DM chromium propionate (KemTRACE Chromium, Kemin Industries); CST + CHR = 13 g per animal daily DM inclusion of prepared Bacillus subtilis PB6 product + 450 ppb DM chromium propionate.

^cItems without common superscript differ (P ≤ 0.05).

^dPooled standard error of the least square mean.