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. Author manuscript; available in PMC: 2021 Jan 15.
Published in final edited form as: Nature. 2020 Jul 15;584(7821):443–449. doi: 10.1038/s41586-020-2548-6

Figure 1. Functional characteristics of neutralizing SARS-CoV-2 mAbs.

Figure 1.

a. Heatmap of mAb neutralization activity, hACE2 blocking activity, and binding to either trimeric S2Pecto protein or monomeric SRBD. MAbs are ordered by neutralization potency, and dashed lines indicate the 12 antibodies with a neutralization IC50 value lower than 150 ng/mL. IC50 values are visualized for viral neutralization and hACE2 blocking, while EC50 values are visualized for binding. The cross-reactive SARS-CoV SRBD mAb rCR3022 is shown as a positive control, while the anti-dengue mAb r2D22 is shown as a negative control. Data are representative of at least 2 independent experiments performed in technical duplicate. No inhibition or no binding indicates an IC50 or EC50 value of >10,000 ng/mL, respectively.

b-d. Correlation of hACE2 blocking, S2Pecto trimer binding, or SRBD binding of mAbs with their neutralization activity. e. Correlation of hACE2 blocking and S2Pecto trimer binding. R2 values are shown for linear regression analysis of log-transformed values. Purple circles indicate mAbs with a neutralization IC50 value lower than 150 ng/mL.

f. Neutralization curves for COV2-2196 and COV2-2130 against authentic SARS-CoV-2 virus. Calculated IC50 values are denoted on the graph. Error bars denote the standard deviation of each point. Data are representative of at least 2 independent experiments, each performed in technical duplicate.

g. Neutralization curves for COV2-2196 and COV2-2130 in a pseudovirus neutralization assay. Error bars denote the standard deviation of each point. Values shown are technical duplicates from a single experiment. Calculated IC50 values from a minimum of 6 experiments are denoted on the graph.

h. hACE2 blocking curves for COV2-2196, COV2-2130, and the non-blocking SARS-CoV mAb rCR3022 in the hACE2 blocking ELISA. Calculated IC50 values are denoted on the graph.. Mean ± SD of technical triplicates are shown from a representative experiment repeated twice.

i. ELISA binding of COV2-2196, COV2-2130, and rCR3022 to trimeric S2Pecto. Calculated EC50 values are denoted on the graph. Mean ± SD of technical triplicates are shown from a representative experiment repeated twice.