Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (M. musculus) | FVB/N | Janvier Labs | FVB/N | FVB ‘WT’ mice |
| Strain, strain background (M. musculus) | C57Bl/6J | Charles River | C57Bl/6J (JAX) | C57Bl/6J ‘WT’ mice |
| Strain, strain background (M. musculus) | OT-I | The Jackson Laboratory (JAX) | Cat 003831 | TCR transgenic mice |
| Strain, strain background (M. musculus) | CD45.1 | The Jackson Laboratory (JAX) | Cat 002014 | CD45.1 congenic marker |
| Cell line (M. musculus) | I3TC | Weiland et al., 2012 | Murine breast cancer cell line derived from MMTV-PyMT FVB mice Mycoplasma test data RATIOS under 1 = clear.I3TC 0.3381995134 |
|
| Cell line (M. musculus) | B16F10 | ATCC | CRL-6475 | Mouse melanoma cell line Mycoplasma test data RATIOS under 1 = clear. B16 OVA 447 163 0.36465324 |
| Cell line (M. musculus) | LLC | ATCC | CRL-1642 | Mouse lung carcinoma cell line Mycoplasma test data RATIOS under 1 = clear. LLC 438 151 0.34474885 |
| Antibody | aSINFENKL | BioLegend | Clone 25-D1.16 | |
| Chemical compound, drug | Sodium L-lactate | Sigma | L7022 | Metabolite |
| Chemical compound, drug | Cyclophosphamide | Sigma | C0768 | Chemotherapy agent |
| Commercial assay or kit | CD8a microbeads | Miltenyi Biotec | 130-117-044 | Beads for magnetic sorting |
| Chemical compound, drug | Collagenase A | Fisher Scientific | 50-100-3278 | For single cell dissociation of tumors |
| Chemical compound, drug | DNase | Sigma | D5025 | For single cell dissociation of tumors |
| Commercial assay or kit | Dynabeads Mouse T-activator | Thermo Fisher | 11456D | Dynabeads Mouse T-activator CD3/CD28 |
| Chemical compound, drug | IL-2 | Sigma | 11011456001 | IL-2 |
| Chemical compound, drug | Citric acid | Sigma | 251275 | |
| Chemical compound, drug | Malic acid | Sigma | M1000 | |
| Chemical compound, drug | Succinic acid | Sigma | S3674 | |
| Chemical compound, drug | Fumaric acid | Sigma | 47910 | |
| Chemical compound, drug | Lactic acid | Sigma | L1750 | |
| Chemical compound, drug | Pyruvic acid | Sigma | 107360 | |
| Chemical compound, drug | a-Ketoglutaric acid | Sigma | K1750 | |
| Chemical compound, drug | Oxaloacetic acid | Sigma | O4126 | |
| Commercial assay or kit | Resazurin reduction assay | Sigma | R7017 | T-cell proliferation assessment |
| Commercial assay or kit | Dead cell stain kit | Thermo Fisher | L10119 | Fixable Near-IR Dead cell Stain Kit |
| Antibody | aCD44 (rat monoclonal) | BD Biosciences | Clone IM7 | 1:2000 |
| Antibody | aCD45.1 (mouse monoclonal) |
BD Biosciences | Clone A20 | 1:200 |
| Antibody | aCD45.2 (mouse monoclonal) |
BD Biosciences | Clone 104 | 1:200 |
| Antibody | CD8 (rat monoclonal) |
BD Biosciences | Clone 53–6.7 | 1:200 |
| Antibody | aCTLA-4 (Armenian hamster monoclonal) | BD Biosciences | Clone UC10-4F10-11 | 1:200 |
| Antibody | aCD62L (mouse monoclonal) |
BioLegend | Clone MEL-14 | 1:200 |
| Antibody | aICOS (Armenian hamster monoclonal) |
BioLegend | Clone C398.4A | 1:200 |
| Antibody | aGzmB (mouse monoclonal) |
Thermo Fisher | Clone GB12 | 1:100 |
| Commercial assay or kit | Counting beads | Thermo Fisher | C36950 | CountBright Absolute Counting Beads |
| Software | FlowJo | Tree Star | Version 8.8.7 | |
| Software | Prism | GraphPad | Version 8 | |
| Software | R | R Core Team | Version 3.6.1 | |
| Antibody | CD3 (rabbit polyclonal) |
Abcam | #ab5690 | IHC 1:100 |
| Antibody | F4/80 (rat monoclonal) |
Abd Serotec | #MCA497 | IHC 1:100 |
| Antibody | Podocalyxin (goat polyclonal) |
R and D Systems | #AF1556 | IHC 1:200 |
| Antibody | PCNA (mouse monoclonal) |
Dako | #M0879 | IHC 1:500 |
| Antibody | GzmB (rabbit polyclonal) |
Abcam | #ab4059 | IHC 1:100 |
| Commercial assay or kit | ABC kit | Vector | PK6100 | Avidin-Biotin peroxidase Complex |
| Commercial assay or kit | DAB | Vector | SK4100 | Diaminobenzidine staining |
| Software | Image J | NIH |