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Primers of target (gpx4a, gpx4b, nrf2, keap1) and endogenous control (β-actin) genes
| Gene | Forward (5′-3′) | Reverse (5′-3′) | Accession No. |
|---|---|---|---|
| β-actin | GCAAGAGAGGTATCCTGACC | CCCTCGTAGATGGGCACAGT | XM_019103102.1 |
| gpx4a | GGAACCAGGAACAAATTCCC | AGATCCTTCTCCACCACGCTTG | FJ656211.1 |
| gpx4b | CTACAAGGCAGAGTTTGACCTC | CTTGGATCGTCCATTGGTCC | FJ656212.1 |
| nrf2 | TTCCCGCTGGTTTACCTTAC | CGTTTCTTCTGCTTGTCTTT | JX462955 |
| keap1 | GCTCTTCGGAAACCCCT | GCCCCAAGCCCACTACA | JX470752 |
