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. 2020 Oct 23;3:612. doi: 10.1038/s42003-020-01348-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Expression of MEF2C and SCD5 in response to the restoration of MSTN detected by Western blotting. MSTN restoration is indicated by the presence of flag-tagged MSTN. GAPDH is the internal control. p3×Flag-MSTN is the MSTN expression plasmid that is tagged by the epitope peptide Flag. siNC and siSCD5 show the treatments with negative control siRNA and siRNA against SCD5, respectively. b qPCR detection of miR222 expression in response to the restoration of MSTN. n = 3 independent experiments. “***” Indicates statistically significant. c Ratios of C16:1/C16:0 and C18:1/C18:0 in response to the restoration of MSTN. n = 3 independent experiments. “***” Indicates statistically significant. d A model for the MSTN regulation upon fatty acid metabolism through the MEF2C/miR222/SCD5-dependent cascade.