Figure 5.

Pharmacological inhibition of adipocyte lipolysis reverses epinephrine (Epi)-induced EGP and impaired glucose uptake during hyperinsulinemic–euglycemic clamps. WT mice fasted 4 h and pretreated 30 min prior with vehicle or 5 mg/kg GS-9667. Mice were infused with either saline or epinephrine (Epi, 1 µg/kg/min) and subjected to a 30 min basal period (− 30 to 0 min) followed by a hyperinsulinemic–euglycemic clamp (0–120 min). The mice then received a bolus of 2-DG, and the clamp was continued for an additional 30 min (120–150 min). (a) Glucose infusion rate during the course of the clamp. Dashed line indicates beginning of steady state (90–120 min). (b) Average glucose infusion rates (GIR) and (c) average blood glucose during steady state. 2-DG uptake in (d) inguinal white adipose tissue (iWAT), (e) epididymal white adipose tissue (eWAT), (f) brown adipose tissue (BAT), (g) gastrocnemius (Gastroc.) muscle, and (h) soleus muscle of vehicle- or GS-9667-treated mice following hyperinsulinemic–euglycemic clamp. N = 4–5. Data are shown as mean ± SEM. *P < 0.05 and **P < 0.01, as indicated. Statistical analyses were performed using two-way ANOVA. All comparisons differing by one variable were made and adjusted for with ANOVA, and all significant comparisons are denoted as such.