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. 2020 Oct 23;10:18177. doi: 10.1038/s41598-020-74866-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of the major components of Acanthopanax senticosus HARMS (chlorogenic acid [CHA], ( +)-syringaresinol di–O–β-D-glucoside [SYG], and a mixture of both [MIX]) on hippocampal brain-derived neurotrophic factor (BDNF) signaling. Analysis of hippocampal (A) BDNF, (B) phospho-tropomyosin receptor kinase B (pTrkB), and (C) phospho-cAMP response element-binding protein (pCREB) protein levels by western blotting. BDNF expression was normalized to β-actin expression, whereas pTrkB and pCREB levels were normalized to those of TrkB and CREB, respectively. Each value is presented as a ratio vs the control (Cont) group. Data are presented as means ± SE; n = 5; *p < 0.05, **p < 0.01 vs Cont group (Dunnett’s t-test). Full-length blots are presented in Supplementary Fig. 1.