Table 1.

Yeast-two hybrid protein–protein interactions.

Baita	TfiA		TfiT
Preyb	TfiA	TfiT	TfiA
−His/−Adec	+++	+++	+++
−Hisc	+++	+++	+++
lacZd (Miller UNITS)	128.7 ± 8.6	60.8 ± 5.9	89.8 ± 7.4
lacZd (fold increase)e	27.4	12.9	47.2

^aBait and ^bprey fusions were constructed in pGBT9 and pGAD424 vectors respectively.

^cYMM plates were supplemented with Met and uracil and lacked either His, or both His and Ade as indicated.

^dlacZ reporter activity was assessed by β-galactosidase assay.

^eFold increase relative to self-activation control for each bait vector.