Figure 3.

Fibroblast-selective DR3-deficiency reduces intestinal fibroblast activation: (A) Immunofluorescent staining of vimentin (green) and αSMA (red) from cecal sections of adoptive transfer Rag^−/−, Rag^−/−Dr3^−/−, or Rag^−/−Dr3^∆Col1a2 recipient mice. White arrows denote myofibroblasts that co-localize vimentin and αSMA at 400× magnification. (B) Total numbers and percentage of myofibroblasts over total vimentin-positive cells per mm² of tissue area were quantitated in an automated fashion by TissueGnostics software and displayed; * indicates p < 0.05, **p < 0.01; ns indicates not significant.