Table 2.
Two-way ANOVAs for the effects of CO2 and N-fertilizer levels, and their interaction on the cytosine methylation percentage in the promoterregion (P1) and codingregion (P2, P3, P2 + P3) of Bt-transgene (P1 + P2 + P3) in the leaves of Bt rice with fused Cry1Ab/Ac during tillering stage, grown under ambient and elevated CO2 with different N-fertilizer levels (F and P values).
| Transgene region | Cytosine methylation patterns | CO2 level (CO2) | N-fertilizer level (N) | CO2 × N | |||
|---|---|---|---|---|---|---|---|
| F-values | P-values | F-values | P-values | F-values | P-values | ||
| Promoter region (P1) | CG (%) | 66.98 | < 0.001 | 7.01 | 0.0096 | 58.31 | < 0.001 |
| CHG (%) | 5.76 | 0.034 | 0.74 | 0.50 | 7.33 | 0.008 | |
| CHH (%) | 11.11 | 0.006 | 5.02 | 0.03 | 15.49 | < 0.001 | |
| Codingregion (P2) | CG (%) | 1.61 | 0.23 | 0.05 | 0.95 | 3.02 | 0.086 |
| CHG (%) | – | – | – | – | – | – | |
| CHH (%) | – | – | – | – | – | – | |
| Codingregion (P3) | CG (%) | 0.18 | 0.68 | 5.15 | 0.02 | 3.37 | 0.07 |
| CHG (%) | 3.08 | 0.11 | 1.68 | 0.23 | 0.002 | 0.99 | |
| CHH (%) | 0.19 | 0.67 | 0.83 | 0.46 | 0.99 | 0.40 | |
| Codingregion (P2 + P3) | CG (%) | 0.66 | 0.43 | 1.79 | 0.21 | 4.93 | 0.03 |
| CHG (%) | 3.08 | 0.11 | 1.68 | 0.23 | 0.002 | 0.99 | |
| CHH (%) | 0.20 | 0.67 | 0.83 | 0.46 | 0.99 | 0.40 | |
| Transgene (P1 + P2 + P3) | CG (%) | 18.64 | 0.001 | 2.24 | 0.15 | 6.72 | 0.01 |
| CHG (%) | 18.38 | 0.001 | 3.63 | 0.06 | 3.57 | 0.06 | |
| CHH (%) | 15.47 | 0.002 | 10.41 | 0.002 | 12.12 | 0.001 | |