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. 2020 Oct 23;10:18196. doi: 10.1038/s41598-020-75374-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The number of minor variants (< 5% of abundance) reconstructed with Nano-Q tool changes linearly with the total number of eligible reads for the pipeline. However, as most minor variants have an abundance < 1%, detection of more variants (if more reads were available), is unlikely to impact the frequency estimation of major variants.