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. 2020 Oct 23;3:603. doi: 10.1038/s42003-020-01343-z

Fig. 3. Vascular defects in global Ptp4a2 knockout mice.

Fig. 3

a Western blot of protein isolated from the Ptp4a2+/− (het), +/+ (wt), or −/− (ko) mouse retina. b Retina whole-mount staining with isolectin B4 and c vascular outgrowth quantification in P6 Ptp4a2+/+ and Ptp4a2−/− mice. Each individual data point represents a mouse (average of two retinas). d Retina whole-mount staining with isolectin B4, and quantification of e vascular density and f branch points in P6 Ptp4a2+/+ and Ptp4a2−/− mice. Each individual data point represents a mouse (average of two retinas). g Retina whole-mount staining with isolectin B4 and high magnification of the vascular front and h quantification of the number of sprouts in P6 Ptp4a2+/+ and Ptp4a2−/− mice. Each n represents an individual mouse. i Retina whole-mount staining with isolectin B4 and quantification for j vascular outgrowth and k deeper plexus vascularization in P9 Ptp4a2+/+ and Ptp4a2−/− mice (Mann–Whitney U test: **p value < 0.01, ***p value < 0.001 ****p value < 0.0001). Each individual data point represents a mouse (average of two retinas). Scale bars: b 500 μm, d, g, i 250 μm. Error bars represent mean ± s.e.m.