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. 2020 Oct 23;3:603. doi: 10.1038/s42003-020-01343-z

Fig. 7. PRL-2 regulates NOTCH-1 cleavage.

Fig. 7

a qPCR analysis in HUVEC cells after CTRL or PTP4A2 siRNA treatment (n = 4 independent experiments; two-way ANOVA: ***p value < 0.001, ****p value < 0.0001). b Western blot analysis on HUVEC cells and quantification after CTRL or PTP4A2 siRNA treatment. c Quantifications of blots shown in b (n = 3 independent experiments; two-way ANOVA: **p value < 0.01, ***p value < 0.001, ns nonsignificant p value > 0.05). d, e Relative mRNA level of HEY2 and HES1, respectively, assessed by qPCR analysis in HUVEC cells treated with CTRL, PTP4A2, or NOTCH1 siRNA and plated overnight on DLL-4-coated plate (n = 3 independent experiments; two-way ANOVA: *p value < 0.05 **p value < 0.01, ***p value < 0.001, ****p value < 0.0001, ns nonsignificant p value > 0.05). f Ptp4a2 qPCR analysis in HUVEC cells after CTRL, PTP4A2, or NOTCH1 siRNA treatment (n = 4 independent experiments; two-way ANOVA: ****p value < 0.0001, ns nonsignificant p value > 0.05). g Schematic representation for PRL-2’s role in the vasculature is depicted in the figure. This schematic was created with images adapted from Servier Medical Art licensed under a Creative Commons Attribution 3.0. Error bars represent mean ± s.e.m.