Fig. 5. Growth of M. pneumoniae strain M129 on serum-free semi-defined medium MCMyco measured by genomic DNA quantification after three culture passages.

Growth is detected when M. pneumoniae is grown on full MCMyco medium (red) and in the same medium after removing both sphingomyelin (SPM) and phosphatidylcholine (PC) (green). In absence of the two phospholipids, M. pneumoniae growth is undetectable. Measurements represent an average of 6 replicates and error bars represent the standard deviations of the 6 replicates.