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. 2020 Oct 23;11(10):908. doi: 10.1038/s41419-020-03095-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — (A) The expression of LUCAT1 was detected by qRT-PCR in HCT116 cells, and cells transfectedwith the CRISPR/Cas9 system differed significantly between the LUCAT1 knockout (LUCAT1-KO) and control (NC) groups in HCT116 and SW620 cells. GAPDH was used as an internal control. (B) Reduction in the proliferation ability of LUCAT1-KO HCT116 and SW620 cells compared with the control (NC) cells by a CCK8 assay. (C) Reduction in colony formation ability of LUCAT1-KO HCT116 and SW620 cells compared with that of the control (NC) cells by a colony formation assay. The bar graph indicates the number of colonies, *p < 0.05. (D) Cell cycle of LUCAT1-KO HCT116 and SW620 cells compared with the control (NC) was analyzed by flow cytometry. The distribution of the cell cycle is shown in the graphs. The results are presented as the mean±s.d. and are representative of at least three independent experiments. (E) Images of xenograft-transplantednude mousemodels (n = 6) and dissected tumors 30 days after injection with LUCAT1-KO SW620 cells, LUCAT1-KO HCT116 cells and their corresponding NC cell lines. (F) Tumor growth curves of LUCAT1-KO HCT116 and SW620 cell and control (NC) cell groups in the xenograft model. (G) Xenograft tumor weight for LUCAT1-KO HCT116 and SW620 cell and control (NC) cell groups in the xenograft model. The results are presented as the mean±s.d. and are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns p > 0.05.