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. 2020 Oct 23;11(10):908. doi: 10.1038/s41419-020-03095-4

Fig. 4. Knockdown of NCL restores MYC expression and cell proliferation in LUCAT1-knockout CRC cells.

Fig. 4

(A) LUCAT1-KO HCT116 and SW620 cells were transfected with the indicated shRNAs, and MYC expression was quantified by qRT-PCR. GAPDH was used as a control. (B) LUCAT1-KO HCT116 and SW620 cells were transfected with the indicated shRNAs, and MYC expression was quantified by a Western blot. GAPDH was used as a control. (C) LUCAT1-KO HCT116 and SW620 cells were transfected with the indicated shRNAs, and the proliferation ability was quantified by a CCK8 assay. (D) LUCAT1-KO HCT116 and SW620 cells were transfected with the indicated shRNAs, and the colony formation ability was quantified by a colony formation assay. The results are presented as the mean ± s.d. and are representative of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.