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. 2020 Jul 31;26:123–135. doi: 10.1016/j.jare.2020.07.015

Fig. 2.

Fig. 2

Inhibition of lncRNA HIF1A-AS2 curbs ox-LDL-induced inflammation in ECs, SMCs and HCAECs. (A) RT-qPCR determination of the expression of lncRNA HIF1A-AS2 in cells transfected with si-HIF1A-AS2-1 or si-HIF1A-AS2-2. (B) The expression of lncRNA HIF1A-AS2 in ECs, SMCs and HCAECs transfected with si-HIF1A-AS2 measured by RT-qPCR. (C) ELISA detection of the expression of TNF-α, IL-1β and IL-6 in cell culture supernatants after lncRNA HIF1A-AS2 downregulation in ECs, SMCs and HCAECs. (D), The protein expression of VCAM-1, ICAM-1, and MCP-1 in ECs, SMCs and HCAECs after inhibiting lncRNA HIF1A-AS2 measured by Western blot analysis. (E) Lipid level in ox-LDL-treated cells following HIF1A-AS2 knockdown analyzed by Oil red O staining (400×). *p < 0.05 vs. cells transfected with si-NC or cells without treatment. #p < 0.05 vs. ox-LDL-treated cells transfected with si-NC. Data (mean ± standard deviation) were obtained from three independent experiments and data between two groups were analyzed using unpaired t test.