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. 2020 Jul 31;26:123–135. doi: 10.1016/j.jare.2020.07.015

Fig. 3.

Fig. 3

Knockdown of lncRNA HIF1A-AS2 alleviates ox-LDL-induced inflammation in ECs, SMCs and HCAECs by inhibiting ATF2. (A) The protein expression of ATF2 determined by Western blot analysis after lncRNA HIF1A-AS2 was silenced in ox-LDL-exposed ECs, SMCs and HCAECs. (B) Measurement of ATF2 protein expression by Western blot analysis in response to downregulation of lncRNA HIF1A-AS2 in ox-LDL-exposed ECs, SMCs and HCAECs. (C) ELISA detection of the expression of TNF-α, IL-1β and IL-6 in EC and SMC culture supernatants exposed to ox-LDL. (D) The protein expression of VCAM-1, ICAM-1, and MCP-1 in ox-LDL-exposed ECs, SMCs and HCAECs measured by Western blot analysis. *p < 0.05 vs. the untreated cells or ox-LDL-exposed cells co-transfected with si-NC and oe-NC. #p < 0.05 vs. the cells ox-LDL-exposed transfected with si-NC or ox-LDL-exposed cells co-transfected with si-HIF1A-AS2 and oe-NC. & p < 0.05 vs. the ox-LDL-exposed cells co-transfected with si-NC and oe-ATF2. Data (mean ± standard deviation) were obtained from three independent cell experiments and comparison among multiple groups was analyzed with one-way analysis of variance.